SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching O08684 from www.uniprot.org...

The NucPred score for your sequence is 0.67 (see score help below)

   1  MSLKSEHRVNTSALQKIAADMSNLIDNLDTRELHFEGEEVEFDTSPGDPK    50
  51  AQEKYIPFSSIYNTQGFKEPNIQTYLSGCPIKAQVLEVERFTSTTRVPSI   100
 101  NLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIPIPTKRHTFRR   150
 151  QNVKEEPREMPSLPRSSENTIQEEQFFGRRKQLEDYLTKILKMPMYRNYH   200
 201  ATTEFLDVSQLSFIHDLGPKGLEGMIMKRSGGHRIPGLNCCGQGRACYRW   250
 251  SKRWLIVKDSFLLYMKPDSGAIAFVLLVDKEFRIKVGRKETETKYGLRID   300
 301  NLSRTLILKCNSYRHARWWGGAIEEFIQKHGSDFLKDHRFGSYAAVHENM   350
 351  LAKWYVNAKGYFEDIANAMEEAAEEIFITDWWLSPEIFLKRPVVEGNRWR   400
 401  LDCILKRKAQQGVRIFIMLYKEVELALGINSEYSKRTLMRLHPNIKVMRH   450
 451  PDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSV   500
 501  KRVTSGLSMGSLAAATMESMESLSLKDNHRSHKNEPILKSVDDVDPKLKG   550
 551  VGKPRKFSKFSLYRQLHRRHLHNSDSVSSIDSASNTGSIRSVQTGVGELH   600
 601  GETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRIPWHDIGSVLHGK   650
 651  AARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQSTAHELRYQVPGAV   700
 701  PAKVQLLRSAADWSAGIKHHEESIHSAYINVIENSKHYIYIENQFFISCA   750
 751  DDKVVFNKVGDAIAQRILKAHREGQRYRVYIVIPRLPGFEGDISTGGGNA   800
 801  LQAIMHFNYRTMCRGENSILGQLKPELGNQWINYISFCGLRTHAELEGNL   850
 851  VTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVP   900
 901  SIMDGKEYQAGCFAQGLRLQCFRLVLGYLSDPSEDLQDPVSDKFFKEIWV   950
 951  STAARNATIYDKVFRCLPNDEVHNLMQLRDFISKPILAKDDPIRAEEELR  1000
1001  KIRGFLVQFPFYFLSEENLLPSVGTKEAIVPMEVWT                1036

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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