 | Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden. |
NucPred
Fetching P12957 from www.uniprot.org...
The NucPred score for your sequence is 0.92 (see score help below)
1 MDDFERRRELRRQKREEMRLEAERLSYQRNDDDEEEAARERRRRARQERL 50
51 RQKEEGDVSGEVTEKSEVNAQNSVAEEETKRSTDDEAALLERLARREERR 100
101 QKRLQEALERQKEFDPTITDGSLSVPSRREVNNVEENEITGKEEKVETRQ 150
151 GRCEIEETETVTKSYQRNNWRQDGEEEGKKEEKDSEEEKPKEVPTEENQV 200
201 DVAVEKSTDKEEVVETKTLAVNAENDTNAMLEGEQSITDAADKEKEEAEK 250
251 EREKLEAEEKERLKAEEEKKAAEEKQKAEEEKKAAEERERAKAEEEKRAA 300
301 EERERAKAEEERKAAEERERAKAEEERKAAEERAKAEEERKAAEERAKAE 350
351 EERKAAEERAKAEKERKAAEERERAKAEEEKRAAEEKARLEAEKLKEKKK 400
401 MEEKKAQEEKAQANLLRKQEEDKEAKVEAKKESLPEKLQPTSKKDQVKDN 450
451 KDKEKAPKEEMKSVWDRKRGVPEQKAQNGERELTTPKLKSTENAFGRSNL 500
501 KGAANAEAGSEKLKEKQQEAAVELDELKKRREERRKILEEEEQKKKQEEA 550
551 ERKIREEEEKKRMKEEIERRRAEAAEKRQKVPEDGVSEEKKPFKCFSPKG 600
601 SSLKIEERAEFLNKSAQKSGMKPAHTTAVVSKIDSRLEQYTSAVVGNKAA 650
651 KPAKPAASDLPVPAEGVRNIKSMWEKGNVFSSPGGTGTPNKETAGLKVGV 700
701 SSRINEWLTKTPEGNKSPAPKPSDLRPGDVSGKRNLWEKQSVEKPAASSS 750
751 KVTATGKKSETNGLRQFEKEP 771
Positively and negatively influencing subsequences are coloured according to the following scale:
(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)
What does the NucPred score mean?
You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper. |
NucPred score threshold | Specificity | Sensitivity |
see above | fraction of proteins predicted to be nuclear that actually are nuclear | fraction of true nuclear proteins that are predicted (coverage) |
0.10 | 0.45 | 0.88 |
0.20 | 0.52 | 0.83 |
0.30 | 0.57 | 0.77 |
0.40 | 0.63 | 0.69 |
0.50 | 0.70 | 0.62 |
0.60 | 0.71 | 0.53 |
0.70 | 0.81 | 0.44 |
0.80 | 0.84 | 0.32 |
0.90 | 0.88 | 0.21 |
1.00 | 1.00 | 0.02 |
Sequences which score >= 0.8 with NucPred and which
are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.) |
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