SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching P21139 from www.uniprot.org...

The NucPred score for your sequence is 0.14 (see score help below)

   1  MAAAPFLKHWRTTFERVEKFVSPIYFTDCNLRGRLFGDSCPVTLSSFLTP    50
  51  ERLPYEKAVQQNFSPAQVGDSFGPTWWTCWFRVELVIPEVWVGKEVHLCW   100
 101  ESDGESLVWRDGEPVQGLTKEGEKTSYVLSERLHAADPRSLTLYVEVACN   150
 151  GLLGAGKGSMIAAPDPEKMFQLSQAKLAVFHRDVHNLLVDLELLLGVAKG   200
 201  LGEDNQRSFQALYTANQMVNICDPAQPETYPAAEALASKFFGQRGGESQH   250
 251  TIHATGHCHIDTAWLWPFKETVRKCARSWSTAVKLMERNTEFTFACSQAQ   300
 301  QLEWVKNQYPGLYAQLQEFACRGQFVPVGGTWVEMDGNLPSGEAMVRQFL   350
 351  QGQNFFLQEFGKMCSEFWLPDTFGYSAQLPQIMQGCGIKRFLTQKLSWNL   400
 401  VNSFPHHTFFWEGLDGSQVLVHFPPGDSYGMQGSVEEVLKTVTNNRDKGR   450
 451  TNHSGFLFGFGDGGGGPTQTMLDRLKRLGNTDGQPRVQLSSPGQLFTALE   500
 501  RDSGQLCTWVGELFLELHNGTYTTHAQLKKGNRECEQILHDVELLSSLAL   550
 551  ARSAQFLYPAVQLQRLWRLLLLNQFHDVVTGSCIQLVAEDAMNYYEDIRS   600
 601  HGNTLLSAAAAALCAGEPGPKGLRHYQHTALEAHRSVGTTQGLVGLTRLA   650
 651  LVTVPSIGYAPAPTPTSLQPLLPQQPVFVMQETDGSVTLDNGIIRVRLDP   700
 701  TGCLTSLVLVASGREAIAEGALGNQFVLFDDVPLYWDAWDVMDYHLETRK   750
 751  PVRGQAGTLAVGAEGGLRGSAWFLLQISPNSRLSQEVVLDVGCPYVRFHT   800
 801  EVHWHETHKFLKVEFPARVRSPQATYEIQFGHLQEADPQQHSWDWARYEV   850
 851  WAHRWIDLSECDFGLALLNNCKYGTSVRGNVLSLSLLRAPKAPDVTADMG   900
 901  RHEFTYALMPHKGSFQEAGVIQAAYNLNFPLLALPAPGPAPDTTWSAFSV   950
 951  SSPAVVLETIKQAEKSHQHRTLVLRLYEAHGSHVDCWLHTSLPVQEATLC  1000
1001  DLLEQRDPTGHLSLQDNRLKLTFSPFQVRSLLLVLQPPAN            1040

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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