SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching P57080 from www.uniprot.org...

The NucPred score for your sequence is 0.90 (see score help below)

   1  MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELA    50
  51  VAFLTAKNAKTPPQEETGYYQTALPGNDRYISVGSQADANVIDLTGDDKD   100
 101  DLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPIE   150
 151  VWRDSRNPYDRKRQEKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNY   200
 201  KPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILK   250
 251  DAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFY   300
 301  GRFLAMGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLH   350
 351  SDNSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLY   400
 401  LDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVD   450
 451  VLQYALEFASSKPVCTSPVDDIDASSSASGPLPSQSLPSTTEQQGPCASD   500
 501  LPGSSSPASGAALPLRSVIHKPFTQSRIPPDLPMHPAPRHITEEELCVLE   550
 551  SCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVH   600
 601  EGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASA   650
 651  YCLMYIDDKAQFLIQEEFNKETGQALVGMETLPPDLRDFVEEDNQRFEKE   700
 701  LEEWDTQLAQRSLQEKLLAAPKLREAEASATTAQAGGADYLEQPSRSDLS   750
 751  KHWKEETLRVIAKASHDLEDKGPETVLQSAIKLEYSRLVKLAQEDTPPET   800
 801  DYRLHHVLVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAK   850
 851  LEMIKPEEVNLEEYEEWHADYKKFRETTMYLITGLENFQRESYIDSLLFL   900
 901  LCAYQNNKELLSKGPYRGHDGELISHYRRECLLKLNEQAAELFESGEDGD   950
 951  VNNGLIIMNEFIVPFLPLLLVDDMEEKDILAVEDMRNRWCSYLGQEMEAN  1000
1001  LQEKLTDFLPKLLDCSTEIKGFHEPPKLPSYSAHELCERFARIMLSLSRT  1050
1051  PADGR                                               1055

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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