SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching P91349 from www.uniprot.org...

The NucPred score for your sequence is 0.89 (see score help below)

   1  MEDNSVLNEDSNLEHVEGQPRRSMSQPVLNVEGDKRTSSTSATQQQVLSG    50
  51  AFSSADVRSIPIIQTWEENKALKTKITILRGELQMYQRRYSEAKEASQKR   100
 101  VKEVMDDYVDLKLGQENVQEKMEQYKLMEEDLLAMQSRIETSEDNFARQM   150
 151  KEFEAQKHAMEERIKELELSATDANNTTVGSFRGTLDDILKKNDPDFTLT   200
 201  SGYEERKINDLEAKLLSEIDKVAELEDHIQQLRQELDDQSARLADSENVR   250
 251  AQLEAATGQGILGAAGNAMVPNSTFMIGNGRESQTRDQLNYIDDLETKLA   300
 301  DAKKENDKARQALVEYMNKCSKLEHEIRTMVKNSTFDSSSMLLGGQTSDE   350
 351  LKIQIGKVNGELNVLRAENRELRIRCDQLTGGDGNLSISLGQSRLMAGIA   400
 401  TNDVDSIGQGNETGGTSMRILPRESQLDDLEESKLPLMDTSSAVRNQQQF   450
 451  ASMWEDFESVKDSLQNNHNDTLEGSFNSSMPPPGRDATQSFLSQKSFKNS   500
 501  PIVMQKPKSLHLHLKSHQSEGAGEQIQNNSFSTKTASPHVSQSHIPILHD   550
 551  MQQILDSSAMFLEGQHDVAVNVEQMQEKMSQIREALARLFERLKSSAALF   600
 601  EEILERMGSSDPNADKIKKMKLAFETSINDKLNVSAILEAAEKDLHNMSL   650
 651  NFSILEKSIVSQAAEASRRFTIAPDAEDVASSSLLNASYSPLFKFTSNSD   700
 701  IVEKLQNEVSELKNELEMARTRDMRSPLNGSSGRLSDVQINTNRMFEDLE   750
 751  VSEATLQKAKEENSTLKSQFAELEANLHQVNSKLGEVRCELNEALARVDG   800
 801  EQETRVKAENALEEARQLISSLKHEENELKKTITDMGMRLNEAKKSDEFL   850
 851  KSELSTALEEEKKSQNLADELSEELNGWRMRTKEAENKVEHASSEKSEML   900
 901  ERIVHLETEMEKLSTSEIAADYCSTKMTERKKEIELAKYREDFENAAIVG   950
 951  LERISKEISELTKKTLKAKIIPSNISSIQLVCDELCRRLSREREQQHEYA  1000
1001  KVMRDVNEKIEKLQLEKDALEHELKMMSSNNENVPPVGTSVSGMPTKTSN  1050
1051  QKCAQPHYTSPTRQLLHESTMAVDAIVQKLKKTHNMSGMGPELKETIGNV  1100
1101  INESRVLRDFLHQKLILFKGIDMSNWKNETVDQLITDLGQLHQDNLMLEE  1150
1151  QIKKYKKELKLTKSAIPTLGVEFQDRIKTEIGKIATDMGGAVKEIRKK    1198

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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