SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q5DTH5 from www.uniprot.org...

The NucPred score for your sequence is 0.90 (see score help below)

   1  MPRRKQQAPRRSAAYVPEEELKAAEIDEEHAEDGGLSLDIQESEFACNEE    50
  51  TEIKEAQSYQNSPVSTATNQDAGYGSPFSEGSDQLAHFKSSSSREEKEES   100
 101  QCPDSVSYPQDSLAQIKAVYANLFSESCWSSLALDLKKSNSATSNNDASQ   150
 151  KESSTPTPTPPTSTASTACTTATTAITSCSTSTSHSSTTSNSSSSGYDWH   200
 201  QAALAKTLQQTSSYGLLPEPSLFSTVQLYRQNNKLYGSVFTGASKFRCKD   250
 251  CSAAYDTLVELTVHMNETGHYRDDNRDKDSEKTKRWSKPRKRSLMEMEGK   300
 301  EDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPLKEPVPAITKLVPS   350
 351  TKKRALQDLASPCSPEPTGVATEVALSESAKDQKTANPYVTPNNRYGYQN   400
 401  GASYTWQFEARKAQILKCMECGSSHDTLQQLTAHMMVTGHFLKVTTSASK   450
 451  KGKQLVLDPVVEEKIQSIPLPPTTHTRLPASSIKKQPDSPAGSVASEEKK   500
 501  EPEKEKEKEKAPPAAGDAERKIKEETEDATEKFEPTALYQYLREEDLDDS   550
 551  PKGGVDILKSLENTVSTAISKAQNGAPSWGGYPSIHAAYQLPGTVKPLQS   600
 601  AVQSVQIQPSYASSVKSLSSTEHNALLHSPGSLTPPPHKSNVSAMEELVE   650
 651  KVTGKVSIKKEERPTEKEKSSPVKAISPVAKENKDLPKTEETGSKPQKKG   700
 701  SDSETGKAKKESTLDAHTPNGTEPLKAKVTNGCGHLGIITDHSPEPSFIN   750
 751  PLSALQSIMNTHLGKVSKPVSPSLDPLAMLYKISNSMLDKPVYPTTPAKQ   800
 801  ADAIDRYYYENSDQPIDLTKSKNKPLVSGVADAVSSPLRESALMDISDMV   850
 851  KNLTGRLTPKSSTPSTVSEKSDADGSSFEEALDELSPVHKRKGRQSNWNP   900
 901  QHLLILQAQFASSLRETAEGKYIMSDLGPQERVHISKFTGLSMTTISHWL   950
 951  ANVKYQLRRTGGTKFLKNLDTGHPVFFCNDCASQFRTASTYVSHLETHLG  1000
1001  FSLKDLSKLPLSQIQEQQSVVSKALTNKTLGPLGSSEEDLGSTFQCKLCN  1050
1051  RTFASKHAVKLHLSKTHGKSPEDHLIYVTELEKQ                  1084

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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