SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q60949 from www.uniprot.org...

The NucPred score for your sequence is 0.88 (see score help below)

   1  MEAITFTARKHPFPNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRL    50
  51  SGQCSKKEPRTKQVRLWVSPSGLRCEPDLEKSQPWDPLICSSIFECKPQR   100
 101  VHKLIHNSHDPSYFACLIKEDAAHRQSLCYVFKADDQTKVPEIISSIRQA   150
 151  GKIARQEELRCPSEFDDTFAKKFEVLFCGRVTVAHKKAPPALIDECIEKF   200
 201  NHVSCGRRTDWEAPTGQPSAPGPRPMRKSFSQPGLRSLAFRKEFQDASLR   250
 251  SSTFSSFDNDIENHLIGGHNVVQPTDMEENRTMLFTIGQSEVYLISPDTK   300
 301  KIALEKNFKEISFCSQGIRHVDHFGFICRECSGGGSGGFHFVCYVFQCTN   350
 351  EALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQGLHKLCERIEGMN   400
 401  SSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRNEQRENELIISFLRCLY   450
 451  EEKQKEHSHTGEPKQTLQVAAENIGSDLPPSASRFRLDSLKNRAKRSLTE   500
 501  SLESILSRGNKARGLQDHSASVDLDSSTSSTLSNTSKELSMGDKEAFPVS   550
 551  ETSFKLLGSSDDLSSDSEGHIAEESALLSPQQAFRRRANTLSHFPVECPA   600
 601  PPEPAQSSPGVSQRKLMRYHSVSTETPHERNVDHLPGGESQGCPGQPSAP   650
 651  PPPRLNPSASSPNFFKYLKHNSSGEQSGNAVPKSVSYRNALRKKLHSSSS   700
 701  VPNFLKFLAPVDENNTCDFKNTNRDFESKANHLGDTDGTPVKTRRHSWRQ   750
 751  QIFLRVATPQKACDSPSRYEDYSELGELPPRSPLEPVCEDGPFGPVQEEK   800
 801  RKTSRELRELWKKAILQQILLLRMEKENQKLQASENDLLNKRLKLDYEEI   850
 851  TPCLKEVTTVWEKMLSTPGRSKIKFDMEKVHSAVGQGVPRHHRGEIWKFL   900
 901  AEQFHLKHPFPSKQQPKDVPYKELLKKLTSQQHAILIDLGRTFPTHPYFS   950
 951  AQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKML  1000
1001  KFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPS  1050
1051  LYAAPWFLTVFASQFPLGFVARVFDMIFLQGSEVIFKVALSLLGSHKPLI  1100
1101  LQHENLETIVDFIKNTLPNLGLVQMEKTISQVFEMDIAKQLQAYEVEYHV  1150
1151  LQEELIESSPLSDNQRMEKLEKTNSSLRKQNLDLLEQLQVANARIQSLEA  1200
1201  TVEKLLTSESKLKQAALTLEVERSALLQMVEELRRQSARPSTPEPDCTQL  1250
1251  EPTGD                                               1255

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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