SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q63085 from www.uniprot.org...

The NucPred score for your sequence is 0.80 (see score help below)

   1  MRKDERERDTPAMRSPPPPPPPATATAASPPESLRNGYVKSCVSPLRQDP    50
  51  PRSFFFHLCRFCNVEPPAASLRAGARLSLAALAAFVLAALLGAGPERWAA   100
 101  AATGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQRGPDRGAGSWWLL   150
 151  ALPACCYLGDFAAWQWWSWLRGEPAAAAAGRLCLVLSCVGLLTLAPRVRL   200
 201  RHGVLVLLFAGLVWWVSFSGLGALPPALRPLLSCLVGGAGCLLALGLDHF   250
 251  FHVRGASPPPRSASTADEKVPVIRPRRRSSCVSLGESAAGYYGSGKMFRR   300
 301  PSLPCISREQMILWDWDLKQWCKPHYQNSGGGNGVDLSVLNEARNMVSDL   350
 351  LIDPSLPPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFYPCSEVEDP   400
 401  VEKGDRKLHKGLSSKPSFPTAQLRRSSGASGLLTSEHHSRWDRSGGKRPY   450
 451  QELSVSSHGCHLNGPFSSNLMTIPKQRSSSVSLTHHAGLRRAGALPSPSL   500
 501  LNSSSHVPVSAGCLTNRSPVGFLDTSDFLTKPSVTLHRSLGSVSSAADFH   550
 551  QYLRNSDSSLCSSCGHQILKYVSTCEPDGTDHHNEKSGEEDSTVFSKERL   600
 601  NIVETQEEETVKEDCRELFLEGDDHLMEEAQQPNIDQEVLLDPMLVEDYD   650
 651  SLIEKMSNWNFQIFELVEKMGEKSGRILSQVMYTLFQDTGLLETFKIPTQ   700
 701  EFMNYFRALENGYRDIPYHNRVHATDVLHAVWYLTTRPIPGLQQLHNNHE   750
 751  TETKADSDARLSSGQIAYLSSKSCCIPDKSYGCLSSNIPALELMALYVAA   800
 801  AMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEY   850
 851  NFLLNLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIE   900
 901  WSSENDRLLVCQVCIKLADINGPAKDRDLHLRWTEGIVNEFYEQGDEEAT   950
 951  LGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWIEAEE  1000
1001  GDDTESDDDDDDDDDDDDDDDEELDSDDEETEDNLNPKPQRRKGRRRIFC  1050
1051  QLMHHLTENHKIWKEIIEEEEKCKAEGNKLQVDNASLPQADEIQVIEEAD  1100
1101  EEEEQMFE                                            1108

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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