SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q63406 from www.uniprot.org...

The NucPred score for your sequence is 0.84 (see score help below)

   1  MSNCWCFIFCKERVRSNSSSPQHDGTSREEADHQVDVSDGIRLVPDKAEA    50
  51  TMATASDEIMHQDIVPLCAADIQEQLKKRFAYLSGGRGQDGSPVITFPDY   100
 101  PAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVL   150
 151  RIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKVPVMMLSSV   200
 201  PELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQA   250
 251  FGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLR   300
 301  EPLAESIVHSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQC   350
 351  LQLRHFEQGFREVKTALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEK   400
 401  SSVAVDKARALSLEGQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRR   450
 451  RDLLSKSLELHSLLETSMKWSDEGIFLLASQPVDKCQSQDGAEAALQEIE   500
 501  KFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRR   550
 551  QASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALR   600
 601  RGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEE   650
 651  LLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRE   700
 701  LESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQE   750
 751  CQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALS   800
 801  SILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKV   850
 851  KELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAV   900
 901  GITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQ   950
 951  ACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEG  1000
1001  CVSSSLPKPPEKGKGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGV  1050
1051  GPRKLVPGKYTVLMDGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTS  1100
1101  SKEGWVPASSLATLLGKSSSAQCLSSSGKTHCARQLCPEPAKILSPEPV   1149

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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