SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q8K4M9 from www.uniprot.org...

The NucPred score for your sequence is 0.69 (see score help below)

   1  MNTEAEQQLLHHARNGNAEEVRKLLEAMARAEVVADIDCKGRSKSNLGWT    50
  51  PLHLACYFGHKQVVQDLLKAGAKVNMLNDMGDTPLHRAAFTGRKELVMLL   100
 101  LEYNADTTVVNGSGQTAKEATHDKEIRQMLEAVERTQQRKLEELLLGAAR   150
 151  EGRTAEVSALLSRPNPPDVNCSDQLGNTPLHCAAYRAHKQCVLKLLRSGA   200
 201  DPSLKNKNDQKPLDLAHGTEMKHILVGNKVVHKALKRFEGPLWKSSRFFG   250
 251  WKLFWVVLEHGVLSWYRKQPDAVHNSYRQGCKHLTQAVCTVKPTDGCLFS   300
 301  VRCFDDTVHGFRVPKNSLQQSREKWLEAIEEHSAYSTHYCSQDQVTDDEE   350
 351  EDVASAMDLKESLARAQTCQQRLDREIYNFLKMIKECDVAKDMLPSFLQK   400
 401  ADVLSEASRETCVALSDCLNLFTKQEGVRNFKLEQEQEKNKILSEALETL   450
 451  ATEHHELERSLVEGSPPVSILSEDEFYDALSGSESEGSLTCLEAVTARAF   500
 501  EENEVPANSGKHRMSEGKDCGGGDALSNGIKKHRTSLPSPMFSRNDFSIW   550
 551  SILRKCIGMELSKITMPVIFNEPLSFLQRLTEYMEHTYLIHKASSFSDPV   600
 601  ERMQCVAAFAVSAVASQWERTGKPFNPLLGETYELVRDDLGFRLISEQVS   650
 651  HHPPISAFHAEGLNNDFIFHGSIYPKLKFWGKSVEAEPKGTITLELLEHN   700
 701  EAYTWTNPTCCVHNIIVGKLWIEQYGNVEIINHKTGDKCVLNFKPCGLFG   750
 751  KELHKVEGYIQDKSKKKLCALYGKWTECLYSVDPATFDAYKKNDKKNTEE   800
 801  KKTSKQASTSEESDEMPVPDSESVFVIPGSALLWRIAPRPPNSAQMYNFT   850
 851  SFAMVLNEVDKEMETVIPKTDCRLRPDIRAMENGEIDQASEEKKRLEEKQ   900
 901  RAARKNRSKSEEDWKTRWFHQGPNPYSGAQDWIYSGSYWDRNYFNLPDIY   950

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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