SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q8N8E3 from www.uniprot.org...

The NucPred score for your sequence is 0.84 (see score help below)

   1  MEVGSEEEKWEKLDAEFDHFVVDMKPFVLKLPHRTERQRCALWIRKLCEP    50
  51  SGTGAGIMGRKNRNLYAKLLLHMLKRGALEGPFTHRPEPGTLKILPSYMS   100
 101  IYFDEPNPARAKGSSPEGLPAWVLGELETSEHKLNESWKLSSGEDNTLVQ   150
 151  SPTDVYSREQYTGKLRVRSHSLSPTHREDGQNITPKICEVYSKKSPVSLD   200
 201  DSDIEARLNSWNLGIENPRYLRQKPIPVSLMTPKFSLRKSSSFHDDHFLS   250
 251  RIREKELDMKTKMMEAKFHEEKLKLQQKHDADVQKILERKNNEIEELKTL   300
 301  YRSKQHETEETIRKLEKKVQTLIRDCQVIRETKEDQIAELKKICEQSTES   350
 351  LNNDWEKKLHNAVAEMEQEKFDLQKQHTENIQELLEDTNVRLNKMESEYM   400
 401  AQTQSTNHMIKELEARVQQLTGEAENSNLQRQKLIQEKAELERCYQITCS   450
 451  ELQEVKARRNTLHKEKDHLVNDYEQNMKLLQTKYDADINLLKQEHALSAS   500
 501  KASSMIEELEQNVCQLKQQLQESELQRKQQLRDQENKFQMEKSHLKHIYE   550
 551  KKAHDLQSELDKGKEDTQKKIHKFEEALKEKEEQLTRVTEVQRLQAQQAD   600
 601  AALEEFKRQVELNSEKVYAEMKEQMEKVEADLTRSKSLREKQSKEFLWQL   650
 651  EDIRQRYEQQIVELKLEHEQEKTHLLQQHNAEKDSLVRDHEREIENLEKQ   700
 701  LRAANMEHENQIQEFKKRDAQVIADMEAQVHKLREELINVNSQRKQQLVE   750
 751  LGLLREEEKQRATREHEIVVNKLKAESEKMKIELKKTHAAETEMTLEKAN   800
 801  SKLKQIEKEYTQKLAKSSQIIAELQTTISSLKEENSQQQLAAERRLQDVR   850
 851  QKFEDEKKQLIRDNDQAIKVLQDELENRSNQVRCAEKKLQHKELESQEQI   900
 901  TYIRQEYETKLKGLMPASLRQELEDTISSLKSQVNFLQKRASILQEELTT   950
 951  YQGRR                                                955

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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