SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q8R516 from www.uniprot.org...

The NucPred score for your sequence is 0.14 (see score help below)

   1  MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDR    50
  51  TVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLR   100
 101  GMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLP   150
 151  RIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVAS   200
 201  VTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRV   250
 251  SADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRIT   300
 301  DRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGH   350
 351  GEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEED   400
 401  ANLDVAERARENKSAASVSVAGSLSVALDKLRTQKSDPEHPGRLVVEAAL   450
 451  GNVARALDLLRRHPEQASYHPALVVDTKNQGRTALQVAAYLGQVELVRLL   500
 501  LQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTA   550
 551  LHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEV   600
 601  LTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDG   650
 651  FTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLV   700
 701  PLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSR   750
 751  LQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLK   800
 801  ALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAEC   850
 851  LVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVV   900
 901  NAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAP   950
 951  CGAALNACPICRQPIRDRIQIFV                              973

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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