SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q91783 from www.uniprot.org...

The NucPred score for your sequence is 0.90 (see score help below)

   1  MSSQNSFMSSKKDDKGKNIQVVVRCRPFNQLERKASSHSVLECESQRKEV    50
  51  CVRTGEVNDKLGKKTYTFDMVFGPAAKQIDVYRSVVCPILDEVIMGYNCT   100
 101  IFAYGQTGTGKTFTMEGERSSDEEFTWEQDPLAGIIPRTLHQIFEKLSEI   150
 151  GTEFSVKVSLLEIYNEELFDLLSPSPDVGERLQMFDDPRNKRGVIIKGLE   200
 201  EISVHNKDEVYQILERGAAKRKTASTLMNAYSSRSHSVFSVTIHMKETTI   250
 251  DGEELVKIGKLNLVDLAGSENIGRSGAVDKRAREAGNINQSLLTLGRVIT   300
 301  ALVERAPHIPYRESKLTRILQDSLGGRTKTSIIATVSPASINLEETMSTL   350
 351  DYASRAKNIMNKPEVNQKLTKKALIKEYTEEIERLKRELATAREKNGVYL   400
 401  SNENYEQLQGKVLSQEEMITEYSEKIAAMEEEIKRIGELFADNKKELEEC   450
 451  TTILQCKEKELEATQNNLQESKEQLAQEAFVVSAMETTEKKLHGTANKLL   500
 501  STVRETTRDVSGLHEKLDRKRAVEQHNSQVHENFAEQINRRFSVIQQTVD   550
 551  EYSVKQQGMLDFYTNSIDDLLGASSSALSATATAVAKSFASVQETVSKQV   600
 601  SHSVEEILKQETLSSQAKDDLQKLMTAHRTGLEQALRTDLLPVVTAVLDL   650
 651  NSHLSHCLQSFLGVADKIDSHKEDMNSFFTEHSRSLHKLRLDSSSALSSI   700
 701  QSEYESLKEEIATAQSTHSEGVNNLISSLQNQLNLLAMETRQQFSGFLSK   750
 751  GGKLQESVGCLQQDLDLVSSDAIECISSHHSKFTEQSQAVTVEIRQLAGS   800
 801  NMSTLEESSKQCEKLTNSINTICQESQQWCESAGQKMDSLLEEQVCYLHS   850
 851  SKKQIQTLHKDVEDGCGSSVVEITDRVNVQCQAQEKALTSLVEQVKDDKE   900
 901  MLGEQRLELNEQVQSGLNKVHVYLKEELRNDVPTGTTPQRRDYVYPSLLI   950
 951  KTKPRDVLLEQFRQQQQEYLESICSVISEAVEPPVEQDSLEDEPPVAVND  1000
1001  SVISEKSCIDLSMTCQEKGGVPFFQQKKALRKEKENRGNATLLERSKIMD  1050
1051  EVEQSLPKSKLPLRMQN                                   1067

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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