SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q9BZA7 from www.uniprot.org...

The NucPred score for your sequence is 0.42 (see score help below)

   1  MDLLSGTYIFAVLLACVVFHSGAQEKNYTIREEMPENVLIGDLLKDLNLS    50
  51  LIPNKSLTTAMQFKLVYKTGDVPLIRIEEDTGEIFTTGARIDREKLCAGI   100
 101  PRDEHCFYEVEVAILPDEIFRLVKIRFLIEDINDNAPLFPATVINISIPE   150
 151  NSAINSKYTLPAAVDPDVGINGVQNYELIKSQNIFGLDVIETPEGDKMPQ   200
 201  LIVQKELDREEKDTYVMKVKVEDGGFPQRSSTAILQVSVTDTNDNHPVFK   250
 251  ETEIEVSIPENAPVGTSVTQLHATDADIGENAKIHFSFSNLVSNIARRLF   300
 301  HLNATTGLITIKEPLDREETPNHKLLVLASDGGLMPARAMVLVNVTDVND   350
 351  NVPSIDIRYIVNPVNDTVVLSENIPLNTKIALITVTDKDADHNGRVTCFT   400
 401  DHEIPFRLRPVFSNQFLLETAAYLDYESTKEYAIKLLAADAGKPPLNQSA   450
 451  MLFIKVKDENDNAPVFTQSFVTVSIPENNSPGIQLTKVSAMDADSGPNAK   500
 501  INYLLGPDAPPEFSLDCRTGMLTVVKKLDREKEDKYLFTILAKDNGVPPL   550
 551  TSNVTVFVSIIDQNDNSPVFTHNEYNFYVPENLPRHGTVGLITVTDPDYG   600
 601  DNSAVTLSILDENDDFTIDSQTGVIRPNISFDREKQESYTFYVKAEDGGR   650
 651  VSRSSSAKVTINVVDVNDNKPVFIVPPSNCSYELVLPSTNPGTVVFQVIA   700
 701  VDNDTGMNAEVRYSIVGGNTRDLFAIDQETGNITLMEKCDVTDLGLHRVL   750
 751  VKANDLGQPDSLFSVVIVNLFVNESVTNATLINELVRKSTEAPVTPNTEI   800
 801  ADVSSPTSDYVKILVAAVAGTITVVVVIFITAVVRCRQAPHLKAAQKNKQ   850
 851  NSEWATPNPENRQMIMMKKKKKKKKHSPKNLLLNFVTIEETKADDVDSDG   900
 901  NRVTLDLPIDLEEQTMGKYNWVTTPTTFKPDSPDLARHYKSASPQPAFQI   950
 951  QPETPLNSKHHIIQELPLDNTFVACDSISKCSSSSSDPYSVSDCGYPVTT  1000
1001  FEVPVSVHTRPPMKEVVRSCTPMKESTTMEIWIHPQPQRKSEGKVAGKSQ  1050
1051  RRVTFHLPEGSQESSSDGGLGDHDAGSLTSTSHGLPLGYPQEEYFDRATP  1100
1101  SNRTEGDGNSDPESTFIPGLKKAAEITVQPTVEEASDNCTQECLIYGHSD  1150
1151  ACWMPASLDHSSSSQAQASALCHSPPLSQASTQHHSPRVTQTIALCHSPP  1200
1201  VTQTIALCHSPPPIQVSALHHSPPLVQATALHHSPPSAQASALCYSPPLA  1250
1251  QAAAISHSSPLPQVIALHRSQAQSSVSLQQGWVQGADGLCSVDQGVQGSA  1300
1301  TSQFYTMSERLHPSDDSIKVIPLTTFTPRQQARPSRGDSPIMEEHPL     1347

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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