SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q9DC04 from www.uniprot.org...

The NucPred score for your sequence is 0.97 (see score help below)

   1  MNRFNGLCKVCSERRYRQITIRRGKDGFGFTICCDSPVRVQAVDSGGPAE    50
  51  RAGLQQLDTVLQLNERPVEHWKCVELAHEIRSCPSEIILLVWRVVPQIKP   100
 101  GPDGGVLRRASCKSTHDLLSPPNKREKNCTHGAPVRPEQRHSCHLVCDSS   150
 151  DGLLLGGWERYTEVGKRSGQHTLPALSRTTTPTDPNYIILAPLNPGSQLL   200
 201  RPVYQEDTIPEEPGTTTKGKSYTGLGKKSRLMKTVQTMKGHSNYQDCSAL   250
 251  RPHIPHSSYGTYVTLAPKVLVFPVFVQPLDLCNPARTLLLSEELLLYEGR   300
 301  NKTSQVTLFAYSDLLLFTKEEEPGRCDVLRNPLYLQSVKLQEGSSEDLKF   350
 351  CVLYLAEKAECLFTLEAHSQEQKKRVCWCLSENIAKQQQLAAPPTERKMF   400
 401  ETEADEKEMPLVEGKGPGAEEPAPSKNPSPGQELPPGQDLPPSKDPSPSQ   450
 451  ELPAGQDLPPSKDPSPSQELPAGQDLPPSKDPSPSQELPVGQDLPPRKDS   500
 501  SGQEAAPGPESPSSEDIATCPKPPQSPETSTSKDSPPGQGSSPTTELPSC   550
 551  QGLPAGQESTSQDPLLSQEPPVIPESSASVQKRLPSQESPSSLGSLPEKD   600
 601  LAEQTISSGEPPVATGAVLPASRPNFVIPEVRLDNAYSQLDGAHGGSSGE   650
 651  DEDAEEGEEGGEGEEDEEDDTSDDNYGDRSEAKRSSLIETGQGAEGGFSL   700
 701  RVQNSLRRRTHSEGSLLQESRGPCFASDTTLHCSDGEGATSTWAIPSPRT   750
 751  LKKELGRNGGSMHHLSLFFTGHRKMSGTDLTECDEASRKRKSKNIAKDMK   800
 801  NKLAIFRRRNESPGAQPASKTDKTTKSFKPTSEEALKWSESLEKLLLHKY   850
 851  GLEVFQAFLRTEFSEENLEFWLACEDFKKVKSQSKMAAKAKKIFAEFIAI   900
 901  QACKEVNLDSYTREHTKENLQSITRGCFDLAQKRIFGLMEKDSYPRFLRS   950
 951  DLYLDLINQKKMSPPL                                     966

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

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