 | Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden. |
NucPred
Fetching Q9N4M4 from www.uniprot.org...
The NucPred score for your sequence is 0.88 (see score help below)
1 MSSSPPARPCCVCFRFRPHEDEKAQKNTFTRWINFHLEEHSSSGRIEDLF 50
51 EDIRDGVLLCHLIEVLTGEALAVHKGRVSKRVHHIANLTTALTVLRRRGL 100
101 ELINNNAADIADGNPRIVLGLIWQIILHFQIETNMILLREWGWAATGTEE 150
151 PTSSAQPEVVVTAPSPTPSSKKSHSKVSSLSGSKTSLASGEKAPSSPLRQ 200
201 RIASFLTPTKKAPKLTAQPVKQSVEQVFLRWINAEIGDLVGGRRVENMDK 250
251 QWRDGILFCALVSRWRPDVISMREVTNANPRDNLELAFNLAHQHLGVRRL 300
301 LAVEDMMIEKPDKRSVITYVSQFVRMFGERSPMQGREQHEIFLAWLEATY 350
351 LLCTRHELNSQECSRIRREFIEHRPLFNTIIVTKVNYDVEELVEIEKKWD 400
401 CIRETLEKYARRSERDLPEPFASIATWIAGAEHILSRPLDLDATDAKKTV 450
451 TMLQKLISEHQKYMEDLPKRQEDFEEAVKHGGLGGRPVAPEFSEPLRARF 500
501 AQIEEENEPRISTLRILTTHYILLQYLQHIDEKIILWRTADSVTLLLRWI 550
551 KEYTQLNAENPQAKCASYINKITLTMANDQNSKLDKEAILNTSNEKTAET 600
601 LKRFESLWIELKLLKVEWVEWETHVSQLEEIIEERRRNGVPPTPEDEQAL 650
651 AVITAGADQLAPKLGASARLSNNQRLDVLTHSFKKLTKTTIKIGGRLVVE 700
701 LEPSTSEQASKISYSWQASDELLKVEMQLRDRIQRDADSSDREQLEYRAE 750
751 TFRNIRDKLIELERLNEIYDNHSRDQVDTPNRNLIRHDMGTIIYGLQAGQ 800
801 YANFVDLSCYAFVYDEYNQVPVNLTENVLSEDYVREIVNRKKAILTRREN 850
851 NEDEIRESIRDMEECDRIIEGWQSTEIEELRAEWNIKLSEFESWHEMMQQ 900
901 VEVLSQTIQTRLDVTVIQTIWILKERSYDIKHSELGGTLRESLEQLATTS 950
951 ETSVNRHLQNLELSNEQDCPDAIEFLEEVGRESVSKLSAAVDDRYIYTLH 1000
1001 VLRTKMELFRRLQNFCDAVKILRSQNTKWNGIKISQIDQVQSEIDTLIVR 1050
1051 LDEEWTQDANQLRAELASIHGSFFQLEFDRLNEKLNMLIHDKDKLRELMV 1100
1101 HRRHYLTAANELITDSKTDLAQRATSSDHPDDILRATDEVTKALDIKGEE 1150
1151 LRRLGELAEMNITDLVVVALILSFRRRQLGSEGEPEVEELRRALREIIAR 1200
1201 PITEPRDVSPDAIVADILRMKDEKKRDEKTIDEIQATTLTDEQRASFAPL 1250
1251 IEDYRRRADRHRIVFEHLVMIYLDWLSRQFDELEDEIGMTIQTSRADDLR 1300
1301 RMNSTEWNKWKTDLANIERQVGPDTKKALSAELADLHRKRDSMEARINKY 1350
1351 LTHSAKINAKLTQFEKWLNAIEEDIEQTERQFEDPERSYRFGSLHEVALA 1400
1401 KQRLVAKLERLNVANKEEVLRLCERYHTIMHKLTPFQTAVGLPLHVSTNL 1450
1451 DRNGPFQSQISVSSIASSELERPESVMSLTSSIGVIPADVAELSPFEAKI 1500
1501 NKLLQKLHIIEDSYLKGPKPIDTVREDVKQLEKYRNRGAEILQQLSTSNI 1550
1551 EDAEKEGLKHRFVLMLNNYDDILRSIENEIRDDNELTAKNQEILAELSNA 1600
1601 EQTLQNSPLEDLDISAELDRLQMQLDLVKVMCNKPRKYVECELIDSSREG 1650
1651 SPQERRRRKKKVMVMVSNTITTIIHVVEERLEASDISRNPDVQQKLVAVK 1700
1701 ENLRELDTTTVTPHPPSIMSPLGTENRNDLEEVKRLAAEIDRAIDTASSM 1750
1751 YEDAPTDEDALKSAIHLLDDQKVTLNHLHVVLEGIPEKNEQDRTDAIDIA 1800
1801 SSVGEKLGNLKSAVEEVYEEVLASTNPVKEDQPLQHIQEVQTTPAEQSNW 1850
1851 DTDEFSRQPPVLSDERIELKTDPSAIDKFEVRSDEDPVPKIIDLFGQLQT 1900
1901 AVNEASPLACEGTDDVDALQVASDKLTKQDRTIRKIHAILDTIDDPVQKP 1950
1951 AIIESLDKIKDQINNARDNINRQIDNLNYNQTPVVVPKESTKTPLNEIED 2000
2001 AVRQASTVVSDELCNTEKLLSARQTLANVKPQVDSVEANVWSNPETIETV 2050
2051 TPILEEYTTLVDNIEKKLANEIEVPTNDPSRQDLVQQLQDVILECEEVVV 2100
2101 NCDNIEKLEESKLKLEKARPLLDQIGDNVEKLSREQSPDTSDAIDALSNV 2150
2151 HQQYNATIMSIDDKIDELKNPEEDTSAADQLISELHVISEMPAVTIDLSM 2200
2201 LNAIEEGLSTLPAHQAENVQAKIDELRQKKEVADQTEQILSDLNAFGDMP 2250
2251 AITLDLDLLKSVEDGIAVLPVEDSERIKAKIVDLRKKKEDADQAEALLQE 2300
2301 LSVISDMPISTLDLNMLQGIEDNLNSLPAEESDEIREKLNELRRRKQESD 2350
2351 QAEALLQELSVISDMPISTLDLNMLQGVEDNLNSLPTEEADKIREKINDL 2400
2401 RRRKQESDLAEALLQELSVIYDMPTSTIDLNMLQGIEDNLNSLPAEESDK 2450
2451 IREKINDLRRRKQESDLAEALLQELSVIYDMPTSTIDLNMLQGIEDNLNS 2500
2501 LPAEESDKIREKINDLRRRKQESDQAEALLQELSVVSDMPASTIDINMLQ 2550
2551 SIEDGLSTLLSEDRSKIQQAIDSLRKKKSDSDLAQHALEALSVQSKLPSV 2600
2601 SINLEELKKLEETLSTVPVEDSKVIRDKIAELKTEKALADHAENYLVELK 2650
2651 KIEDMPISAVGSDVLATIEDQILQMPVQYQPSVKETLDKLKQAKEEDDKL 2700
2701 AGVYDELEKIAKLPARDYDNKLLAKIDEKLNSLPKDQIAETHRKVEDIKV 2750
2751 TKADIVAQIDVLDKLPAKDIDEHLLNSIEEKLPTIPSDSSDQLQIAIGKL 2800
2801 RDRKQANIDEGKKILNELAEIQKMPADSLNEHALNLLATESDKFGSEISD 2850
2851 KIMQEIDVLREKQNNHEVARLNAESVLQQLDKISEEPHLSLTEERLAPFL 2900
2901 QNIDTVPACFVDKIRNKINEVQKLHDEAVQDEKDELKEKLVAKVQNIGKT 2950
2951 SIDDVNVSDFEEIEREINGSLEAFEAEPLLAKIQELREAKRVGDEARSAA 3000
3001 HDQIVALEKEAEDVTAKESAKKKKKDKKKSPQEMIDELSAKVVEAKALIP 3050
3051 KIEEAAKNENLPADDKPKAEQLVSNLEAFVKDVETQVSEKQDELDKLNNA 3100
3101 NDAIKRLGDALDDAEKTVVPSSVPALSEFKDRIAPHLATLVEAVNDVPAS 3150
3151 VEPSAVALRDRAAKFVSDLEKNIQKTGDDEKRADELKNDVGNAVKNVEDV 3200
3201 VSKYQNQPQPLDVAKDDANKLKATVEQLTKLAESSDKIDPQVAKDIKDSK 3250
3251 TKAKELLQALEKAIPQEDAIRREQAEINDRLNNLEKELTKVDEFKPEDAL 3300
3301 PIVDQLAANTNTLKTATDSNNEKAVAPSSLISHDDLVVGLPEKVFQLQHA 3350
3351 IDDKKQALNKAAAVNEIAPKLQLVSQQLQSVPQEVPASLDEQKQLLEDVE 3400
3401 NQKHNLENLLANLPENDPTADELRQKSQWDLSRLKDLLKQLGSAVGDKLA 3450
3451 ALAAFNAARKNAEDALLDITREDGGDDNKSPDELIDDLAKKEETVAKLLD 3500
3501 TVSGVKPDELDDKERAEYNDLLARLATAADVLKNKRAELEQAVKAKADEK 3550
3551 SLHDSVDRIVSRLVPLVRESDELRHNAEAVPTQYAPKAEELKKEVEAAKA 3600
3601 VIANAPSSDAHVQQLEQAVATAETLIPDLEERARLWNEFLAARNDIDALI 3650
3651 EQLQQPLDAVLAQPKRSAEEAAQDVENLRNNSQQLSDLDNKIANLQRISE 3700
3701 LLDPLESAYADVRFFDVDAEQTRHQYDDVLNDVAAELEDETLLKQSASQV 3750
3751 ANEIDDISKMIDSTDPERSILDTIAKSDIPALKAQINRIKDRIVNADASR 3800
3801 KHVTTDPKIAEDLDNKLAKLQTELDDAIKTSDEHDKEQLILSLKLNISQF 3850
3851 EQIPLDQLKSDDLKTAEKEITNSLKPEEAEPLLAKIQELREAKRVGDEAR 3900
3901 SAAHDQIVALEKEAEDVTAKESAKKKKKDKKKSPQEMIDELSAKVVEAKA 3950
3951 LIPKIEEAAKNENLPADDKPKAEQLVSNLEAFVKDVETQVSEKQDELDKL 4000
4001 NNANDAIKRLGDALDDAEKTVVPSSVPALSEFKDRIAPHLATLVEAVNDV 4050
4051 PASVEPSAVALRDRAAKFVSDLEKNIQKTGDDEKRADELKNDDGNAVKNV 4100
4101 EDVVSKYQNQPQPLDVAKDDANKLKATVEQLTKLAESSDKIDPQVAKDIK 4150
4151 DSKTKAKELLQALEKAIPQEDAIRREQAEINDRLNNLEKELTKVDEFKPE 4200
4201 DALPIVDQLAANTNTLKTATDSNNEKAVAPSSLISHDDLVVGLPEKVFQL 4250
4251 QHAIDDKKQALNKAAAVNEIAPKLQLVSQQLQSVPQEVPASLDEQKQLLE 4300
4301 DVENQKHNLENLLANLPENDPTADELRQKSQWDLSRLKDLLKQLGSAVGD 4350
4351 KLAALAAFNAARKNAEDALLDITREDGGDDNKSPDELIDDRGRSTGSAVG 4400
4401 DKLAALAAFNAARKNAEDALLDITREDGGDDNKSPDELIDDLAKKEETVA 4450
4451 KLLDTVSGVKPDELDDKERAEYNDLLARLATAADVLKNKRAELEQAVKAK 4500
4501 ADEKSLHDSVDRIVSRLVPLVRESDELRHNAEAVPTQYAPKAEELKKEVE 4550
4551 AAKAVIANAPSSDAHVQQLEQAVATAETLIPDLEERARLWNEFLAARNDI 4600
4601 DALIEQLQQPLDAVLAQPKRSAEEAAQDVENLRNNSQQLSDLDNKIANLQ 4650
4651 RISELLDPLESAYADVRFFDVDAEQTRHQYDDVLNDVAAELEDETLLKQS 4700
4701 ASQVANEIDDISKMIDSTDPERSILDTIAKSDIPALKAQINRIKDRIVNA 4750
4751 DASRKHVTTDPKIAEDLDNKLAKLQTELDDAIKTSDEHDKEQLILSLKLN 4800
4801 ISQFEQIPLDQLKSDDLKTAEKEITNSLKPEEAEPLLAKIQELREAKRVG 4850
4851 DEARSAAHDQIVALEKEAEDVTAKESAKKKKKDKKKSPQEMIDELSAKVV 4900
4901 EAKALIPKIEEAAKNENLPADDKPKAEQLVSNLEAFVKDVETQVSEKQDE 4950
4951 LDKLNNANDAIKRLGDALDDAEKTVVPSSVPALSEFKDRIAPHLATLVEA 5000
5001 VNDVPASVEPSAVALRDRAAKFVSDLEKNIQKTGDDEKRADELKNDVGNA 5050
5051 VKNVEDVVSKYQNQPQPLDVAKDDANKLKATVEQLTKLAESSDKIDPQVA 5100
5101 KDIKDSKTKAKELLQALEKAIPQEDAIRREQAEINDRLNNLEKELTKVDE 5150
5151 FKPEDALPIVDQLAANTNTLKTATDSNNEKAVAPSSLISHDDLVVGLPEK 5200
5201 VFQLQHAIDDKKQALNKAAAVNEIAPKLQLVSQQLQSVPQEVPASLDEQK 5250
5251 QLLEDVENQKHNLENLLANLPENDPTADELRQKSQWDLSRLKDLLKQLGS 5300
5301 AVGDKLAALAAFNAARKNAEDALLDITREDGGDDNKSPDELIDDLAKKEE 5350
5351 TVAKLLDTVSGVKPDELDDKERAEYNDLLARLATAADVLKNKRAELEQAV 5400
5401 KAKADEKSLHDSVDRIVSRLVPLVRESDELRHNAEAVPTQYAPKAEELKK 5450
5451 EVEAAKAVIANAPSSDAHVQQLEQAVATAETLIPDLEERARLWNEFLAAR 5500
5501 NDIDALIEQLQQPLDAVLAQPKRSAEEAAQDVENLRNNSQQLSDLDNKIA 5550
5551 NLQRISELLDPLESAYADVRFFDVDAEQTRHQYDDVLNDVAAELEDETLL 5600
5601 KQSASQVANEIDDISKMIDSTDPERSILDTIAKSDIPALKAQINRIKDRI 5650
5651 VNADASRKHVTTDPKIAEDLDNKLAKLQTELDDAIKTSDEHDKEQLILSL 5700
5701 KLNISQFEQIPLDQLKSDDLKTAEKEITNSLKPEEAEPLLAKIQELREAK 5750
5751 RVGDEARSAAHDQIVALEKEAEDVTAKESAKKKKKDKKKSPQEMIDELSA 5800
5801 KVVEAKALIPKIEEAAKNENLPADDKPKAEQLVSNLEAFVKDVETQVSEK 5850
5851 QDELDKLNNANDAIKRLGDALDDAEKTVVPSSVPALSEFKDRIAPHLATL 5900
5901 VEAVNDVPASVEPSAVALRDRAAKFVSDLEKNIQKTGDDEKRADELKNDV 5950
5951 GNAVKNVEDVVSKYQNQPQPLDVAKDDANKLKATVEQLTKLAESSDKIDP 6000
6001 QVAKDIKDSKTKAKELLQALEKAIPQEDAIRREQAEINDRLNNLEKELTK 6050
6051 VDEFKPEDALPIVDQLAANTNTLKTATDSNNEKAVAPSSLISHDDLVVGL 6100
6101 PEKVFQLQHAIDDKKQALNKAAAVNEIAPKLQLVSQQLQSVPQEVPASLD 6150
6151 EQKQLLEDVENQKHNLENLLANLPENDPTADELRQKSQWDLSRLKDLLKQ 6200
6201 LGSAVGDKLAALAAFNAARKNAEDALLDITREDGGDDNKSPDELIDDLAK 6250
6251 KEETVAKLLDTVSGVKPDELDDKERAEYNDLLARLATAADVLKNKRAELE 6300
6301 QAVKAKADEKSLHDSVDRIVSRLVPLVRESDELRHNAEAVPTQYAPKAEE 6350
6351 LKKEVEAAKAVIANAPSSDAHVQQLEQAVATAETLIPDLEERARLWNEFL 6400
6401 AARNDIDALIEQLQQPLDAVLAQPKRSAEEAAQDVENLRNNSQQLSDLDN 6450
6451 KIANLQRISELLDPLESAYADVRFFDVDAEQTRHQYDDVLNDVAAELEDE 6500
6501 TLLKQSASQVANEIDDISKMIDSTDPERSILDTIAKSDIPALKAQINRIK 6550
6551 DRIVNADASRKHVTTDPKIAEDLDNKLAKLQTELDDAIKTSDEHDKEQLI 6600
6601 LSLKLNISQFEQIPLDQLKSDDLKTAEKEITNSLKPEEAEPLLAKIQELR 6650
6651 EAKRVGDEARSAAHDQIVALEKEAEDVTAKESAKKKKKDKKKSPQEMIDE 6700
6701 LSAKVVEAKALIPKIEEAAKNENLPADDKPKAEQLVSNLEAFVKDVETQV 6750
6751 SEKQDELDKLNNANDAIKRLGDALDDAEKTVVPSSVPALSEFKDRIAPHL 6800
6801 ATLVEAVNDVPASVEPSAVALRDRAAKFVSDLEKNIQKTGDDEKRADELK 6850
6851 NDVGNAVKNVEDVVSKYQNQPQPLDVAKDDANKLKATVEQLTKLAESSDK 6900
6901 IDPQVAKDIKDSKTKAKELLQALEKAIPQEDAIRREQAEINDRLNNLEKE 6950
6951 LTKVDEFKPEDALPIVDQLAANTNTLKTATDSNNEKAVAPSSLISHDDLV 7000
7001 VGLPEKVFQLQHAIDDKKQALNKAAAVNEIAPKLQLVSQQLQSVPQEVPA 7050
7051 SLDEQKQLLEDVENQKHNLENLLANLPENDPTADELRQKSQWDLSRLKDL 7100
7101 LKQLGSAVGDKLAALAAFNAARKNAEDALLDITREDGGDDNKSPDELIDD 7150
7151 LAKKEETVAKLLDTVSGVKPDELDDKERAEYNDLLARLATAADVLKNKRA 7200
7201 ELEQAVKAKADEKSLHDSVDRIVSRLVPLVRESDELRHNAEAVPTQYAPK 7250
7251 AEELKKEVEAAKAVIANAPSSDAHVQQLEQAVATAETLIPDLEERARLWN 7300
7301 EFLAARNDIDALIEQLQQPLDAVLAQPKRSAEEAAQDVENLRNNSQQLSD 7350
7351 LDNKIANLQRISELLDPLESAYADVRFFDVDAEQTRHQYDDVLNDVAAEL 7400
7401 EDETLLKQSASQVANEIDDISKMIDSTDPERSILDTIAKSDIPALKAQIN 7450
7451 RIKDRIVNADASRKHVTTDPKIAEDLDNKLAKLQTELDDAIKTSDEHDKE 7500
7501 QLILSLKLNISQFEQIPLDQLKSDDLKTAEKEITNSLKPEEAEPLLAKIQ 7550
7551 ELREAKRVGDEARSAAHDQIVALEKEAEDVTAKESAKKKKKDKKKSPQEM 7600
7601 IDELSAKVVEAKALIPKIEEAAKNENLPADDKPKAEQLVSNLEAFVKDVE 7650
7651 TQVSEKQDELDKLNNANDAIKRLGDALDDAEKTVVPSSVPALSEFKDRIA 7700
7701 PHLATLVEAVNDVPASVEPSAVALRDRAAKFVSDLEKNIQKTGDDEKRAD 7750
7751 ELKNDVGNAVKNVEDVVSKYQNQPQPLDVAKDDANKLKATVEQLTKLAES 7800
7801 SDKIDPQVAKDIKDSKTKAKELLQALEKAIPQEDAIRREQAEINDRLNKL 7850
7851 EKELTKVDEFKPEDALPIVDQLAANTNTLKTATDSNNEKAVAPSSLISHD 7900
7901 DLVVGLPEKVFQLQHAIDDKKQALNKAAAVNEIAPKLQLVSQQLQSVPQE 7950
7951 VPASLDEQKQLLEDVENQKHNLENLLANLPENDPTADELRQKSQWDLSRL 8000
8001 KDLLKQLGSAVGEKLAALAAFNAARKNAEDALLDITREDGGDDNKSPDEL 8050
8051 IDDLAKKEETVAKLLDTVSGVKPDELDDKERAEYNDLLARLATAADVLKN 8100
8101 KRAELEQAVKAKADEKSLHDSVDRIVSRLVPLVRESDELRHNAEAVPTQY 8150
8151 APKAEELKKEVEAAKAVIANAPSSDAHVQQLEQAVATAETLIPDLEERAS 8200
8201 IWERFVKAKDDLYDYLEKLENNVSDVLNRPRLPVSQAQQRFNKLKEQSYL 8250
8251 LDRIRDLKIDFDDLGEALLPLTVAEDELRFMHVHVESIERQYEDTMDKLN 8300
8301 AEITAEVELLRTLDILSNELSQCKEDINNPSVDVDELSRATMLNDAIAHL 8350
8351 ENQKVVVARSEKDRKFVESSTSIDLDQLLAEAKRLLKEIEPRLQLAQPDH 8400
8401 DNEDDEDEEKGSDEKPYDVRAAAEVLSALYPDEHPHNVLRNIGFEELPSD 8450
8451 SESRSEFDSLDSRSDGLLSPIPDDSTLSEEQLRRQRSRWRRVLRTALPLQ 8500
8501 ALLVLLMGAACLVPHCDDEYCCQLLNNFAKSFDPSLEFVNGPPPF 8545
Positively and negatively influencing subsequences are coloured according to the following scale:
(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)
What does the NucPred score mean?
| You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper. |
| NucPred score threshold | Specificity | Sensitivity |
| see above | fraction of proteins predicted to be nuclear that actually are nuclear | fraction of true nuclear proteins that are predicted (coverage) |
| 0.10 | 0.45 | 0.88 |
| 0.20 | 0.52 | 0.83 |
| 0.30 | 0.57 | 0.77 |
| 0.40 | 0.63 | 0.69 |
| 0.50 | 0.70 | 0.62 |
| 0.60 | 0.71 | 0.53 |
| 0.70 | 0.81 | 0.44 |
| 0.80 | 0.84 | 0.32 |
| 0.90 | 0.88 | 0.21 |
| 1.00 | 1.00 | 0.02 |
| Sequences which score >= 0.8 with NucPred and which
are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.) |
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