SBC logo Authors: Amine Heddad, Andrea Krings, Markus Brameier and Bob MacCallum, Stockholm Bioinformatics Center, Stockholm University, Sweden.

NucPred

Fetching Q9P7C7 from www.uniprot.org...

The NucPred score for your sequence is 0.82 (see score help below)

   1  MSRRTRSRSPPRRSYNRERRDYRKYDDSEDQKGARYNRYVDDVSSRRDRH    50
  51  DSFRSHESNKIRRDNSWKHDKYSYEKRYQERDREYARSKNIPDQYIGRSP   100
 101  RPTSHRHAEEKEVDNKTKSDETNPVLQGSATEIKPQPRRSRFDRTERVGA   150
 151  SLSVSEIQSENPRVDVIPKDKAVENNHQRNAEKPVASDKITDAKLLARLE   200
 201  RVRAWKESKAKQEASKKEEHKLNTKPQVTAKDQNAMPSTGISGFEINRQK   250
 251  DTSDMKRNNRVHMDDEDGPRRMNLEDYQELWDQEDRGMLGNEQAASMEED   300
 301  EVDPLDAYMASLVGTTDTIRPGLLNTEVIDPNANDDERMVISETLEEEEN   350
 351  LLALAAKRSKKKDVITVDHSKINYEDFKKDFYVEPEELKNLSPAEVDELR   400
 401  ASLDGIKIRGIDCPKPVTSWSQCGLSAQTISVINSLGYEKPTSIQAQAIP   450
 451  AITSGRDVIGVAKTGSGKTIAFLLPMFRHIKDQRPLKTGEGPIAIIMTPT   500
 501  RELAVQIFRECKPFLKLLNIRACCAYGGAPIKDQIADLKRGAEIVVCTPG   550
 551  RMIDVLSANAGRVTNLHRCTYLVLDEADRMFDLGFEPQVMRIINNIRPDR   600
 601  QTVLFSATFPRAMEALARKVLKKPVEITVGGRSVVASEVEQIVEVRPEES   650
 651  KFSRLLELLGELYNNQLDVRTLVFVDRQESADALLSDLMKRGYTSNSIHG   700
 701  GKDQHDRDSTISDYKAGVFDVLIATSVVARGLDVKSLQLVVNYDCPNHME   750
 751  DYVHRVGRTGRAGHTGVAVTFITPEQEKYAVDIAKALKMSKQPVPKELQT   800
 801  LASQFLEKVKAGKEKAAGGGFGGKGLSRLDETRNAERKMQRKAYGEDEED   850
 851  VETEAEAKSPLEKITPEKSTGDPTLDRVRAAVGGIAARAFANQTAQSNKL   900
 901  TQPISIIKTDGDEYKAKMEINDYPQQARWAVTNNTNIVHVTELTGTSITT   950
 951  KGNFYLPGKNPEPGEEKLYLWIEGPSELVVNRAITELRRLLLEGINHSLE  1000
1001  GGNKPSASGRYTVV                                      1014

Positively and negatively influencing subsequences are coloured according to the following scale:

(non-nuclear) negative ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| positive (nuclear)

with NucPred



If you find NucPred useful, please cite this paper:
NucPred - Predicting Nuclear Localization of Proteins. Brameier M, Krings A, Maccallum RM. Bioinformatics, 2007. PubMed id: 17332022
The authors also look forward to your comments and suggestions.

What does the NucPred score mean?

You have to decide on a NucPred score threshold. Sequences which score greater than or equal to this threshold are predicted to spend some time in the nucleus. Higher thresholds yield fewer predicted nuclear proteins, but these predictions are more accurate (you can have higher confidence in them). The table below gives more details of the performance of NucPred estimated using the sequences it was trained on (by cross-validation). Another benchmark is available in the Bioinformatics 2007 paper.

NucPred score threshold Specificity Sensitivity
see above fraction of proteins predicted to be nuclear that actually are nuclear fraction of true nuclear proteins that are predicted (coverage)
0.10 0.45 0.88
0.20 0.52 0.83
0.30 0.57 0.77
0.40 0.63 0.69
0.50 0.70 0.62
0.60 0.71 0.53
0.70 0.81 0.44
0.80 0.84 0.32
0.90 0.88 0.21
1.00 1.00 0.02

Sequences which score >= 0.8 with NucPred and which are predicted by PredictNLS to contain an NLS have been shown to be 93% correct with a coverage of 16%. (PredictNLS by itself is 87% correct with 26% coverage on the same data.)

Go back to the NucPred Home Page.